Journal: Cancer Immunology, Immunotherapy : CII

Article Title: FUBP3 mediates MXI1 stability to silence RRAS and hinder MAPK signaling in acute megakaryoblastic leukemia progression

doi: 10.1007/s00262-025-04257-z

Figure Lengend Snippet: Knockdown of RRAS blocks the MAPK signaling to slow AMKL in vivo. ( A ) WBC count in the peripheral blood of NOG mice injected with M-07e cells infected with sh-NC or sh- RRAS #2 (n = 5, unpaired t-test). ( B ) The spleen and liver tissues of mice injected with M-07e cells infected with sh-NC or sh- RRAS #2 (n = 5, unpaired t-test). ( C ) Knockdown efficiency of RRAS in the spleen tissues of AMKL mice detected by immunohistochemistry (n = 5, unpaired t-test). ( D ) Representative HE images of bone marrow, liver, and spleen sections of mice (n = 5, unpaired t-test). ( E ) Detection of CD41, a surface marker of megakaryocytes, in single-cell suspensions from mouse spleens by flow cytometry (n = 5, unpaired t-test). ( F ) The percentage of CD8 + T cells in single-cell suspensions from mouse spleen by flow cytometry (n = 5, unpaired t-test). ( G ) Detection of CD8 in the spleen tissues of mice by immunofluorescence staining (n = 5, unpaired t-test). ( H ) Detection of IFN-γ + CD8 + in single-cell suspensions from mouse spleens by flow cytometry (n = 5, unpaired t-test). ( I ) Expression of GzmB + CD8 + and PRF1 + CD8 + in single-cell suspensions of mouse spleen detected by flow cytometry (n = 5, unpaired t-test). Data were presented as means ± SEM, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Human peripheral blood monocytes (CP-H182) and human AMKL cell lines M-07e (CL-0686) and MEG-01 (CL-0498) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Knockdown, In Vivo, Injection, Infection, Immunohistochemistry, Marker, Flow Cytometry, Immunofluorescence, Staining, Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: FUBP3 mediates MXI1 stability to silence RRAS and hinder MAPK signaling in acute megakaryoblastic leukemia progression

doi: 10.1007/s00262-025-04257-z

Figure Lengend Snippet: Knockdown of MXI1 upregulates RRAS and activates the MAPK signaling to suppress the anti-tumor immunity of CD8 + T cells. ( A ) WBC counts in the peripheral blood of NOG mice injected with M-07e cells infected with oe-NC, oe- FUBP3 , oe- FUBP3 + sh-NC, or oe- FUBP3 + sh- MXI1 #2 (n = 5, one-way ANOVA). ( B ) The spleen and liver tissues of mice injected with M-07e cells (n = 5, one-way ANOVA). ( C ) The positive rate of FUBP3, MXI1, and RRAS in the spleen tissues of AMKL mice was detected by immunohistochemistry (n = 5, one-way ANOVA). ( D ) The phosphorylation and total protein of ERK-1/2 in the spleen tissues of AMKL mice were detected by western blot analysis (n = 3, one-way ANOVA). ( E ) Representative HE images of bone marrow, liver, and spleen sections of mice (n = 5, one-way ANOVA). ( F ) Detection of CD41 and CD8 in single-cell suspensions from mouse spleens by flow cytometry (n = 5, one-way ANOVA). ( G ) Detection of CD8 in the spleen tissues of mice by immunofluorescence staining (n = 5, one-way ANOVA). ( H ) Detection of IFN-γ + CD8 + in single-cell suspensions from mouse spleens by flow cytometry (n = 5, one-way ANOVA). ( I ) Expression of GzmB + CD8 + and PRF1 + CD8 + in single-cell suspensions of mouse spleen detected by flow cytometry (n = 5, one-way ANOVA). Data were presented as means ± SEM, one-way ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Human peripheral blood monocytes (CP-H182) and human AMKL cell lines M-07e (CL-0686) and MEG-01 (CL-0498) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Knockdown, Injection, Infection, Immunohistochemistry, Phospho-proteomics, Western Blot, Flow Cytometry, Immunofluorescence, Staining, Expressing